Invitrogen GL7 Monoclonal Antibody (GL-7 (GL7)), PerCP-eFluor™ 710, 25 µg, eBioscience™
On demandInvitrogen GL7 Monoclonal Antibody (GL-7 (GL7)), PerCP-eFluor™ 710, 25 µg, eBioscience™
This GL7 monoclonal antibody reacts with a cell-surface protein found on T and B lymphocytes activated in vitro, on bone marrow pre-B-II cells, germinal center B cells, and also human B cell lines Ramos and Daudi. There is strain variability with respect to antigen distribution on thymocytes and Con A-activated spleen cells, with expression in BALB/c greater than that in C57BL/6. GL7 is commonly used as a marker for mouse germinal center B cells. The epitope of GL7 has been identified as a sialic acid glycan moiety called Neu5Ac. This moiety is recognized by CD22.
PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser
(488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend
using a 710/50 bandpass filter, however, the 695/40 bandpass filter is
an acceptable alternative. Please make sure that your instrument is
capable of detecting this fluorochrome.
Light
sensitivity: This tandem dye is sensitive to photo-induced oxidation.
Please protect this vial and stained samples from light.
Fixation:
Samples can be stored in IC Fixation Buffer (100 µL of
cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution
for up to 3 days in the dark at 4°C with minimal impact
on brightness and FRET efficiency/compensation. Some generalizations
regarding fluorophore performance after fixation can be made, but clone
specific performance should be determined empirically.
Filtration: 0.2 µm post-manufacturing filtered.
Applications: Functional Assay (FN).
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