Invitrogen™ Glutathione Reductase Fluorescent Activity Kit

Invitrogen™ Glutathione Reductase Fluorescent Activity Kit

The Glutathione Reductase Activity research-use-only kit is a fluorescent activity assay designed for the quantification and detection of glutathione reductase activity in serum, plasma, RBCs and cell lysates.

This complete, ready-to-use kit includes black 96-well plate(s), glutathione reductase standard (200 mU/mL), glutathione reductase substrate, and other components to perform the assay. A 96-well microplate reader capable of reading the fluorescent emission at 510 nm, with excitation at 390 nm, is required for use of this kit.

Features

• Sample types: serum, plasma, RBCs, and cell lysates
• Product Type: Glutathione S-Transferase Fluorescent Activity Kit
• Sensitivity: 0.009 mU/mL
• Standard curve range: 0.15 mU/mL–5 mU/mL
• Reactivity: human
• Storage: Store at –20°C.
• Shipping Condition: Approved for shipment on Wet or Dry Ice
• Target Kit Named: Glutathione Reductase
• For Use With (Equipment): Microplate Reader
• Immunoassay Kit Format: Activity Kit

Content

• Glutathione Reductase Standard; 200 U/mL glutathione reductase in a special stabilizing solution, 40 μL
• Assay Buffer Concentrate (2X), 60 mL
• Black 96-well Half Area Plate, 1 plate
• Detection Reagent; r econstitute with Dry DMSO, 1 vial
• Dry DMSO (dimethyl sulfoxide), 2 mL
• NADPH; reduced ß-nicotinamide adenine dinucleotide 2’-phosphate freeze dried with stabilizers stored in a desiccator, 1 vial
• NADPH Diluent; phosphate buffer containing detergents and stabilizers, 5 mL
• Oxidized Glutathione; oxidized glutathione (GSSG) in special stabilizing solution, 3 mL