Invitrogen™ Amplex™ Red/UltraRed Stop Reagent
On demandInvitrogen™ Amplex™ Red/UltraRed Stop Reagent
Amplex™ Red/UltraRed Stop Reagent is designed for use with the Amplex™ Red and Amplex™ UltraRed fluorogenic substrates and assay kits. Amplex™ Red/UltraRed Stop Reagent provides convenience and control by allowing the fluorescence signal-generating reaction to be terminated at a user-determined time point. After addition of the stop reagent, the fluorescence signal remains stable for at least 3 hours.
Amplex™ UltraRed and Amplex™ Red assay kits are sensitive biomolecular
assays based on hydrogen peroxide–generating enzyme systems linked to
peroxidase–mediated oxidation of the fluorogenic Amplex™ UltraRed or
Amplex™ Red substrates. Typically, detection reactions are performed in
microplate wells and are initiated by adding the fluorogenic Amplex™
UltraRed or Amplex™ Red substrate, resulting in a continuous
fluorescence increase that proceeds for 30 minutes or more. Ultimately,
unknown analyte concentrations are determined by comparing fluorescence
intensities measured at a certain time point during the reaction to
parallel measurements at the same time point on standard samples of
known concentration. Clearly, it is critical to ensure that the timing
of the standard and unknown sample measurements is the same.
Amplex™
Red/UltraRed Stop Reagent is designed for use with both the Amplex™ Red
and Amplex™ UltraRed fluorogenic substrates and assay kits. It is
designed to terminate reactions containing up to 0.1 units/mL of
horseradish peroxidase (HRP) and 5 μM hyrogen peroxide. The fluorescent
signal then remains stable for at least 3 hours
There are no specifications
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