Invitrogen GM-CSF Human ELISA Kit, Biotin conjugated Antibody, 96 Tests

Invitrogen GM-CSF Human ELISA Kit,  Biotin conjugated Antibody, 96 Tests

The Human Granulocyte Macrophage-Colony Stimulating Factor (Hu GM-CSF) ELISA quantitates Hu GM-CSF in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu GM-CSF.
Principle of the method
The Human GM-CSF solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Features  

• Homogenous (no wash): No    
• Interassay CV: 4.8%
• Intraassay CV: 5.7%
• Storage: 2-8°C
• Shipping condition: Wet Ice
  
  

Contents    

• Pre-coated 96 well plate
• Standard
• Standard Dilution Buffer
• Assay Buffer concentrate
• Biotinylated Detection Antibody
• SAV-HRP
• HRP Diluent
• Wash Buffer
• Chromogen
• Stop Solution
• Adhesive Plate Covers