Invitrogen GM-CSF Human Instant ELISA™ Kit, 128 Tests
On demandInvitrogen GM-CSF Human Instant ELISA™ Kit, 128 Tests
The Human Granulocyte Macrophage Colony Stimulating Factor (Hu GM-CSF)
ELISA quantitates Hu GM-CSF in human serum, plasma, cell culture
supernatants, or other body fluids. The assay will exclusively recognize
both natural and recombinant Hu GM-CSF.
Principle of the method
The
Human GM-CSF solid-phase sandwich ELISA (enzyme-linked immunosorbent
assay) is designed to measure the amount of the target bound between a
matched antibody pair. A target-specific antibody has been pre-coated in
the wells of the supplied microplate. Samples or controls are then
added into these wells and bind to the immobilized (capture) antibody.
The sandwich is formed by the binding of the second (detector) antibody
to the target on a different epitope from the capture antibody. An
antibody conjugated with enzyme binds the formed sandwich. After
incubation and washing steps to rid the microplate of unbound
substances, a substrate solution is added that reacts with the
enzyme-antibody-target complex to produce measurable signal. The
intensity of this signal is directly proportional to the concentration
of target present in the original specimen.
Features
- Homogenous (no wash): No
- Interassay CV: 13.5%
- Intraassay CV: 5.9%
- Storage: -20°C
Contents
- Pre-coated 96 well Microplate
- Biotinylated Detection Antibody
- SAV-HRP
- Sample Diluent, lyophilized
- Standard (8-well strips x 4)
- Wash Buffer Concentrate
- Substrate Solution
- Sample Diluent
- Stop Solution
- Lysis Buffer 10x
- Adhesive Plate Covers
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