Invitrogen Growth Hormone Rat ELISA Kit, 96 Tests

Invitrogen Growth Hormone Rat ELISA Kit, 96 Tests

The Rat Growth Hormone ELISA Kit quantifies rat growth hormone in serum, plasma, buffered solution, or cell culture medium. The assay will recognize both the natural and recombinant growth hormone.
Principle of the method
The rat growth hormone solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Features  

• Homogenous (no wash): No    
• Interassay CV: 5%
• Intraassay CV: 4.8%
• Shipping condition: Wet Ice
• Storage: 2-8°C

Contents    

• Rt Growth Hormone Standard, lyophilized; contains 0.1% sodium azide. Refer to vial label for reconstitution volume, 2 vials
• Standard Diluent Buffer; contains 0.1% sodium azide, 25 mL
• Rt Growth Hormone Antibody Coated Plate, 96-well plate, 1 plate
• Rt Growth Hormone Biotin Conjugate; contains 0.1% sodium azide, 11 mL
• Streptavidin‑HRP (100X), 0.15 mL
• Streptavidin‑HRP Diluent, 25 mL
• Wash Buffer Concentrate (25X), 100 mL
• Stabilized Chromogen, Tetramethylbenzidine (TMB), 25 mL
• Stop Solution, 25 mL
• Adhesive Plate Covers, 4