Invitrogen HLA-DR Monoclonal Antibody (LN3), Super Bright 600, 25 Tests, eBioscience™
On demand
Order number :
63-9956-41
Invitrogen HLA-DR Monoclonal Antibody (LN3), Super Bright 600, 25 Tests, eBioscience™
The LN3 mAb reacts with the human major histocompatibility complex (MHC) class II, HLA-DR. HLA-DR is expressed on the surface of human antigen presenting cells (APC) including B cells, monocytes, macrophages, DCs, and activated T cells. HLA-DR is a heterodimeric transmembrane protein composed of alpha and beta subunits and plays an important role in the presentation of peptides to CD4+ T lymphocytes.
Features
- When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.
- Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Protect this vial and stained samples from light.
- Fixation: Samples can be stored in IC Fixation Buffer (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution for up to 3 days in the dark at 2-8°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorochrome performance after fixation can be made, but clone specific performance should be determined empirically.
- Excitation: 405 nm; Emission: 600 nm; Laser: Violet Laser.
- Recommended Isotype Control: Mouse IgG2b kappa Isotype Control (eBMG2b), Super Bright 600, eBioscience™.
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