Invitrogen HLA-DR Monoclonal Antibody (LN3), Super Bright 702, 100 Tests, eBioscience™

Invitrogen HLA-DR Monoclonal Antibody (LN3), Super Bright 702, 100 Tests, eBioscience™

The LN3 mAb reacts with the human major histocompatibility complex (MHC) class II, HLA-DR. HLA-DR is expressed on the surface of human antigen presenting cells (APC) including B cells, monocytes, macrophages, DCs, and activated T cells. HLA-DR is a heterodimeric transmembrane protein composed of alpha and beta subunits and plays an important role in the presentation of peptides to CD4+ T lymphocytes.

Features  

• Super Bright 645 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 645 nm. We recommend using a 660/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome. 
• When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information. 
• Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. 
• Fixation: Samples can be stored in IC Fixation Buffer (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. 
• Excitation: 405 nm; Emission: 645 nm; Laser: Violet Laser. 
• Recommended Isotype Control: Mouse IgG2b kappa Isotype Control (eBMG2b), Super Bright 702, eBioscience™.