Invitrogen IFN gamma Canine ELISA Kit, 96 Tests

Invitrogen IFN gamma Canine ELISA Kit, 96 Tests

The Canine Inteferon gamma (Dog IFNγ) ELISA quantitates Dog IFNγ in canine serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Dog IFNγ.
Principle of the method
The Canine IFNγ solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

• Homogenous (no wash): No    
• Interassay CV: <12%
• Intraassay CV: <10%

Contents    

• Canine IFN-gamma Antibody Coated wells, 96-well plate, 1 plate
• Canine IFN-gamma Biotin Conjugate, 2 vials
• Canine IFN-gamma Standard, recombinant canine IFN-gamma, 2 vials
• Wash Buffer Concentrate (20X), 25 mL
• Assay Diluent (5X), 15 mL
• Streptavidin-HRP (300X), 0.2 mL
• TMB Substrate, 12 mL
• Stop Solution, 8 mL
• Adhesive Plate Covers, 2