Invitrogen HVEM/TNFRSF14 Human ELISA Kit, 10 x 96 Tests, 100 µL

Invitrogen HVEM/TNFRSF14 Human ELISA Kit, 10 x 96 Tests, Sample Volume 100 µL 

The Human Herpes Virus Entry Mediator (HVEM/TNFSF14) ELISA quantitates Hu HVEM in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu HVEM.

Principle of the method

The Human HVEM solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Features 

• Homogenous (no wash): No
• Interassay CV: <12%
• Intraassay CV: <10%
• Shipping condition: Wet or Dry Ice
• Storage: 2-8°C

Contents

• Human TNFRSF14 Antibody Coated wells, 96 well plate, 1 plate
• Human TNFRSF14 Biotin Conjugate, 2 vials
• Human TNFRSF14 Standard, recombinant human TNFRSF14, 2 vials
• Wash Buffer Concentrate (20X), 25 mL
• Assay Diluent C, 30 mL
• Assay Diluent B (5X concentrated buffer), 15 mL
• Streptavidin-HRP (200X), 0.125 mL
• TMB Substrate, 12 mL
• Stop Solution, 8 mL
• Adhesive Plate Covers, 2