Invitrogen HVEM/TNFRSF14 Human ELISA Kit, 96 Tests, 50 µL

Invitrogen HVEM/TNFRSF14 Human ELISA Kit, 96 Tests, Sample Volume 50 µL

The Human Tumor Necrosis Factor Receptor Super Family 14 (Hu TNFRSF14)/HerpesVirus Entry Mediator (HVEM), ELISA quantitates Hu HVEMin human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu HVEM.

Principle of the method

The Human HVEM solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Features 

• Homogenous (no wash): No
• Interassay CV: 8.2%
• Intraassay CV: 4.8%
• Storage: 2-8°C

Contents

• Pre-coated 96 well plate
• Standard
• Sample Diluent
• Assay Buffer concentrate
• Biotinylated Detection Antibody
• SAV-HRP
• Wash Buffer
• Controls
• Chromogen
• Stop Solution
• Adhesive Plate Covers