Invitrogen IFN gamma Bovine ELISA Kit, 96 Tests
On demandInvitrogen IFN gamma Bovine ELISA Kit, 96 Tests
The Bovine Interferon gamma (Bov IFN-gamma) ELISA semi-quantitates Bov IFN-gamma in plasma or cell culture medium. The assay will exclusively recognize natural Bov IFNγ.
Principle of the method
The Bov IFNγ solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
- Homogenous (no wash): No
- Interassay CV: 2.6%
- Intraassay CV: 3%
Contents
- Pre-coated 96 well plate
- Standard
- Standard Dilution Buffer
- Incubation Buffer
- HRP conjugated Detection Antibody
- Wash Buffer
- Chromogen
- Stop Solution
- Controls
There are no specifications
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