Invitrogen STAT3 (Phospho) [pY705] Human InstantOne™ ELISA Kit, 96 Tests

Invitrogen STAT3 (Phospho) [pY705] Human InstantOne™ ELISA Kit, 96 Tests

Representative data for STAT3 (Phospho) [pY705] Multispecies InstantOne™ ELISA. A431 cells were pre-treated with 10 µM AG1478 (an EGFR kinase inhibitor), washed and unstimulated (-) or stimulated with 10 ng/mL EGF for 10 min (+). Cells were analyzed by immunoblotting (left) and InstantOne™ ELISA (right). The intensity of the band at 89 kDa as detected by immunoblot correlates with InstantOne™ ELISA absorption values.

Features

• Sample type/volume: Cell Lysate, 20 µL
• Hands-on time: 1 hr 20 min
• Time-to-result: 1 hr 10 min
• Homogenous (no wash): No
• Instrument: Colorimetric Microplate Reader
• Product size: 96 Tests
• Shipping condition: Wet Ice
• Storage: 2-8°C

Contents

• User Manual
• STAT3 (Total) Capture Antibody Reagent (Part No. IOSTAT3CAR1) - 3 mL
• STAT3 (Total) Detection Antibody Reagent (Part No. IOSTAT3DAR1) - 3 mL
• InstantOne™ Assay Plate (Part No. IOAP96) - 96 well (sealed)
• Cell Lysis Buffer (5X) (Part No. IOCLB1) - 12 mL
• Enhancer Solution (Part No. IOES1) - 1 mL
• Wash Buffer (10X) (Part No. IOWB1) - 15 mL
• Detection Reagent (TMB Substrate) (Part No. IODR1) - 12 mL
• Stop Solution (Part No. IOSS1) - 12 mL
• Positive Control Cell Lysate (lyophilized) (Part No. IOSTAT3PC1) – 0.25 mL
• Plate seal - 1 ea