Invitrogen IFN gamma Human ELISA Kit, Biotin conjugated Antibody, 2 x 96 Tests, Sinsitivity <4 pg/mL

Invitrogen IFN gamma Human ELISA Kit, Biotin conjugated Antibody, 2 x 96 Tests, Sinsitivity <4 pg/mL

The Human Interferon-gamma (Hu IFN-γ) ELISA quantitates Hu IFN-γ in human serum, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IFN-γ.
Principle of the method
The Human IFN-γ solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

• Homogenous (no wash): No    
• Interassay CV: 6.0%
• Intraassay CV: 5.5%

Contents    

• Hu IFN-g Standard, lyophilized; contains 0.1% sodium azide, 2 vials
• Standard Diluent Buffer; contains 0.1% sodium azide, 25 mL
• Antibody Coated Wells; 96-well plate, 1 plate
• Incubation Buffer; contains 8 mM sodium azide, 12 mL
• Hu IFN-g Biotin Conjugate (Biotin-labeled anti-IFN-g); contains 0.1% sodium azide, 6 mL
• Streptavidin-HRP (100X), 0.15 mL
• Streptavidin-HRP Diluent; contains 3.3 mM thymol, 25 mL
• Wash Buffer Concentrate (25X), 100 mL
• Stabilized Chromogen, Tetramethylbenzidine (TMB), 25 mL
• Stop Solution, 25 mL
• Plate Covers, adhesive strips, 3