Invitrogen IGFBP-2 Mouse ELISA Kit, 10 x 96 Tests

Invitrogen IGFBP-2 Mouse ELISA Kit, 10 x 96 Tests

The Mouse IGF Binding Protein 2 (Ms IGFBP-2) ELISA quantitates Ms IGFBP-2 in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms IGFBP-2.
Principle of the method
The Mouse IGFBP-2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

• Homogenous (no wash): No    
• Interassay CV: <12%
• Intraassay CV: <10%

Contents    

• Mouse IGFBP-2 Antibody Coated wells, 96-well plate, 10 plate
• Mouse IGFBP-2 Biotin Conjugate, 20 vials
• Mouse IGFBP-2 Standard, recombinant mouse IGFBP- 2, 20 vials
• Wash Buffer Concentrate (20X), 12 x 25 mL
• Assay Diluent C, 10 x 30 mL
• Assay Diluent B (5X), 10 x 15 mL
• Streptavidin-HRP (500X), 10 x 0.2 mL
• TMB Substrate, 10 x 12 mL
• Stop Solution, 10 x 8 mL
• Adhesive Plate Covers, 20