Invitrogen IL-1 beta Bovine Uncoated ELISA Kit, 5 x 96 Tests

Invitrogen IL-1 beta Bovine Uncoated ELISA Kit, 5 x 96 Tests

The Bovine IL-1beta Uncoated ELISA Kit contains pre-matched antibody pairs and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of bovine IL-1beta.
Principle of the method
ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

• Homogenous (no wash): No

Contents    

• Coating Antibody
• Lyophilized Recombinant Standard
• Detection Antibody
• Streptavidin-HRP
• Substrate Solution
• Ready to use Stop Solution
• 0.16M Sulfuric Acid
• Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards