Invitrogen IL-17A Human ELISA Kit, High Sensitivity, 96 Tests

Invitrogen IL-17A Human ELISA Kit, High Sensitivity, 96 Tests

The Human High Sensitivity Interleukin 17A (HS IL-17A) ELISA quantitates Hu HS IL-17A in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu HS IL-17A.

Principle of the method

The Human HS IL-17A solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A special amplification reagent step is added to achieve the high sensitivity capability. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Features 

• Homogenous (no wash): No
• Interassay CV: 6.3%
• Intraassay CV: 8.5%
• Storage: 2-8°C

Contents

• Pre-coated 96 well plate
• Standard
• Sample Diluent
• Assay Buffer concentrate
• Biotinylated Detection Antibody
• Amplification Diluent Concentrate
• Amplification Reagent I
• Amplification Reagent II
• SAV-HRP
• Wash Buffer
• Chromogen
• Stop Solution
• Adhesive Plate Covers