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Invitrogen IL-1R1/IL-1RA Human ELISA Kit, 96 Tests

On demand
Order number : EHIL1R1
(Ex-work price)
$ On demand

Invitrogen IL-1R1/IL-1RA Human ELISA Kit, 96 Tests


The Human Interleukin 1 Receptor 1 (Hu IL-1R1/IL-1RA) ELISA quantitates Hu IL-1R1 in human serum, plasma,, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-1R1.

Principle of the method

The Human IL-1R1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.


Features 

  • Homogenous (no wash): No
  • Interassay CV: <12%
  • Intraassay CV: <10%
  • Shipping condition: Wet or Dry Ice
  • Storage: 2-8°C

Contents

  • Human IL-1R1 Antibody Coated wells, 96-well plate, 1 plate 
  • Human IL-1R1 Biotin Conjugate, 2 vials 
  • Human IL-1R1 Standard, recombinant human IL-1R1, 2 vials 
  • Wash Buffer Concentrate (20X), 25 mL 
  • Assay Diluent A (contains 0.09% sodium azide), 30 mL 
  • Assay Diluent B (5X), 15 mL
  • Streptavidin-HRP (200X), 0.2 mL 
  • TMB Substrate, 12 mL 
  • Stop Solution, 8 mL 
  • Adhesive Plate Covers, 2 
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