Invitrogen™ Immune Monitoring 65-Plex Human ProcartaPlex™ Panel for MAGPIX

Invitrogen™ Immune Monitoring 65-Plex Human ProcartaPlex™ Panel for MAGPIX

The Human Immune Monitoring 65-plex ProcartaPlex Panel for MAGPIX is a preconfigured multiplex immunoassay kit that measures 65 cytokines, chemokines, and growth factors using Luminex xMAP technology. Analyze these 65 targets simultaneously for efficient immune response profiling, biomarker discovery, and validation. This kit consists of two separate kits: a 43-plex and a 22-plex kit that have been specifically designed to be used on the MAGPIX instrument that is capable of detecting up to 50 targets simultaneously. Luminex 100 and FLEXMAP 3D users should use the Immune-Monitoring 65 plex Panel (Cat. No. EPX650-10065-901) that contains all 65 targets in a single panel. The panels (43-plex and 22-plex) are provided in a ready-to-use format with individual vials of capture and detection reagents formulated at 1X concentration, requiring less pipetting and experimental setup.
ProcartaPlex preconfigured panels are extensively tested for analyte combinability, interference, and cross-reactivity to provide the highest level of validation and precision. All ProcartaPlex panels are supplied with the necessary reagents to perform the assay.

About ProcartaPlex assays for the Luminex platform, ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

Target list [bead region]:

• 22-plex: APRIL [52], BAFF [65], BLC (CXCL13) [15], CD30 [34], ENA-78 (CXCL5) [55], Eotaxin-2 (CCL24) [37], Eotaxin-3 (CCL26) [77], FGF-2 [75], Fractalkine (CX3CL1) [39], IL-16 [47], IL-2R (CD25) [22], IL-20 [26], I-TAC (CXCL11) [46], MCP-2 (CCL8) [18], MCP-3 (CCL7) [66], MDC (CCL22) [30], MIF [67], MIG (CXCL9) [54], TNF-RII [45], TRAIL (CD253) [29], TSLP [61], TWEAK [35].
• 43-plex: CD40L (CD154) [74], Eotaxin (CCL11) [33], Gro-alpha (CXCL1) [61], G-CSF (CSF-3) [42], GM-CSF [44], HGF [46], IFN alpha [48], IP-10 (CXCL10) [22], IFN gamma [43], IL-1 alpha [62], IL-1 beta [18], IL-2 [19], IL-3 [73], IL-4 [20], IL-5 [21], IL-6 [25], IL-7 [26], IL-8 (CXCL8) [27], IL-9 [52], IL-10 [28], IL-12p70 [34], IL-13 [35], IL-15 [65], IL-17A (CTLA-8) [36], IL-18 [66], IL-21 [72], IL-22 [76], IL-23 [63], IL-27 [14], IL-31 [37], LIF [15], M-CSF [67], MCP-1 (CCL2) [51], MIP-1 alpha (CCL3) [12], MIP-1 beta (CCL4) [47], MIP-3 alpha (CCL20) [56], MMP-1 [64], NGF beta [55], SDF-1 alpha (CXCL12) [13], SCF [39], TNF alpha [45], TNF beta [54], VEGF-A [78].

Features

• Reproducible, reliable results—validated as a panel to the highest industry standard, including protein target combinability and cross-reactivity testing.
• More results per sample—measure multiple protein targets simultaneously in a single 25–50 µL sample.
• Well-established Luminex technology highly referenced multiplexing platform for protein detection and quantitation.

Contents

• 4 vials Human Standard Mix M (lyophilized)
• 1 vial Capture Bead Mix M1 (1 X)
• 1 vial Capture Bead Mix M2 (1 X)
• 1 vial Biotinylated Detection Antibody Mix M1 (1 X)
• 1 vial Biotinylated Detection Antibody Mix M2 (1 X)
• 4 vials Human Control-High
• 4 vials Human Control-Low
• 1 Bottle Reading Buffer (1 X)
• 1 Bottle Wash Buffer (10 x)
• 1 Bottle Streptavidin-PE (1 X)
• 1 Bottle Universal Assay Buffer (1 X)
• 8-tube strip
• Adhesive film
• 2 Flat-bottom 96-well plate, black