Invitrogen™ Cytokine/Chemokine/Growth Factor Convenience 45-Plex Human ProcartaPlex™ Panel 1

Invitrogen™ Cytokine/Chemokine/Growth Factor Convenience 45-Plex Human ProcartaPlex™ Panel 1

The Human Cytokine/Chemokine/Growth Factor Convenience 45-Plex ProcartaPlex Panel 1 enables the study of immune function by analyzing 45 protein targets in a single well using Luminex xMAP technology. The panel is provided in a ready-to-use format with individual vials of capture and detection reagents formulated at 1X concentration, requiring less pipetting and experimental setup. The same targets can be found in the Human Cytokine/Chemokine/Growth Factor 45-Plex ProcartaPlex Panel 1 (Cat. No. EPX450-12171-901) that provides a modular format allowing for more flexibility to increase or decrease the number of targets.
ProcartaPlex preconfigured panels are extensively tested for analyte combinability, interference, and cross-reactivity to provide the highest level of validation and precision. All ProcartaPlex panels are supplied with the necessary reagents to perform the assay.

About ProcartaPlex assays for the Luminex platform, ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.

The Luminex beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create spectrally unique signatures that can be identified by the Luminex xMAP detection systems (e.g., Luminex 200, FLEXMAP 3D, and MAGPIX). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.
The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

Target list [bead region]:

• Th1/Th2: GM-CSF [44], IFN gamma [43], IL-1 beta [18], IL-2 [19], IL-4 [20], IL-5 [21], IL-6 [25], IL-8 [27], IL-12p70 [34], IL-13 [35], IL-18 [66], TNF alpha [45].
• Th9/Th17/Th22/Treg: IL-9 [52], IL-10 [28], IL-17A (CTLA-8) [36], IL-21 [72], IL-22 [76], IL-23 [63], IL-27 [14].
• Inflammatory cytokines: IFN alpha [48], IL-1 alpha [62], IL-1RA [38], IL-7 [26], IL-15 [65], IL-31 [37], TNF beta [54].
• Chemokines: Eotaxin (CCL11) [33], GRO alpha (CXCL1) [61], IP-10 (CXCL10) [22], MCP-1 (CCL2) [51], MIP-1 alpha (CCL3) [12], MIP-1 beta (CCL4) [47], RANTES (CCL5) [42], SDF-1 alpha [13].
• Growth factors: BDNF [57], EGF [56], FGF-2 [75], HGF [46], NGF beta [55], PDGF-BB [77], PlGF-1 [29], SCF [39], VEGF-A [78], VEGF-D [53].

Features

• Reproducible, reliable results—validated as a panel to the highest industry standard, including protein target combinability and cross-reactivity testing.
• More results per sample—measure multiple protein targets simultaneously in a single 25–50 µL sample.
• Well-established Luminex technology highly referenced multiplexing platform for protein detection and quantitation.

Contents

• 2 vials Human Standard Mix A (lyophilized)
• 2 vials Human Standard Mix B (lyophilized)
• 2 vials Human Standard Mix C (lyophilized)
• 1 vial Capture Bead Mix (1X)
• 1 vial Biotinylated Detection Antibody Mix (1X)
• Streptavidin-PE (SA-PE) (1X) 1 x 5 mL
• Wash Buffer (10X) 1 x 25 mL
• Reading Buffer (1X) 1 x 40 mL
• Universal Assay Buffer (1X) 1 x 10 mL
• 8-Tube Strip 2 each
• Flat Bottom 96-well Plate, black 1 each
• Microplate Lid 1 each
• Plate Seals 8 each