Invitrogen IR (Phospho) [pY1162/pY1163] Human ELISA Kit

Invitrogen IR (Phospho) [pY1162/pY1163] Human ELISA Kit

The Human Insulin Receptor beta subunit (Hu IRβ) ELISA quantitates Hu IRβ in human cell and tissue lysates. The assay will exclusively recognize both natural and recombinant Hu IRβ. Cross-reactivity has been observed in mouse and rat cells.

Principle of the method

The Human IRβ solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Features

• Homogenous (no wash): No
• Interassay CV: 7.6%
• Intraassay CV: 6%
• Shipping condition: Wet Ice
• Storage: 2-8°C

Contents

• IR [pYpY1162/1163] Standard, lyophilized; contains 0.1% sodium azide. Refer to vial label for quantity and reconstitution volume, 2 vials
• Standard Diluent Buffer; contains 0.1% sodium azide, 25 mL
• IR (β‑subunit) Antibody Coated Plate, 96-well strip-well plate, 1 plate
• IR [pYpY1162/1163] Detection Antibody; contains 0.1% sodium azide, 11 mL
• Anti‑Rabbit IgG HRP (100X); contains 3.3 mM thymol, 0.125 mL
• HRP Diluent; contains 3.3 mM thymol, 25 mL
• Wash Buffer Concentrate (25X), 100 mL
• Stabilized Chromogen, Tetramethylbenzidine (TMB), 25 mL
• Stop Solution, 25 mL
• Plate Covers, adhesive strips, 3