Invitrogen™ LIVE/DEAD™ Fixable Near-IR Dead Cell Stain Kit, for 633 or 635 nm excitation, 400 Assays

Invitrogen™ LIVE/DEAD™ Fixable Near-IR Dead Cell Stain Kit, for 633 or 635 nm excitation, 400 Assays

The LIVE/DEAD™ Fixable Near-IR Dead Cell Stain Kit is used to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining or prior to elimination of biohazardous materials using formaldehyde fixation. This kit has been optimized and validated for use with a red laser flow cytometer.

Features

• Stable, Unlike products that are sold in solution, the LIVE/DEAD™ Fixable Near-IR Stain has been conveniently packaged in 40-test vials to help ensure the stability and performance of the dye over time. Amine reactive dyes in solution will lose their effectiveness over a short period of time, therefore it is recommended to completely use the vial once rehydrated. If this is not possible, aliquot the vials in small volumes and store at -80°C, avoiding freeze-thaw cycles.
• Robust, Dead cell discriminator stains can lose sensitivity after treatment with fixatives such as formaldehyde or ethanol-based fixation methods required for intracellular phosphorylation studies. The LIVE/DEAD™ Fixable Near-IR Stain is an amine reactive dye that binds covalently to intracellular and extracellular amines, and the staining pattern is preserved following formaldehyde fixation.
• Optimal brightness, The LIVE/DEAD™ Fixable Near-IR stain was selected based on its fluorescent properties to provide a bright signal when excited with a red laser. The near-IR fluorescent reactive dye has an excitation maximum of ∼633 nm making it ideal for use with the red or HeNe laser with an emission of ∼750 nm. Since live and dead cells can be discriminated using a single dye and a single channel of a flow cytometer, it is an ideal choice for multi-color experiments.

Content

• 10 vials of LIVE/DEAD™ fixable dead cell stain and 500 μL DMSO.