Invitrogen™ LIVE/DEAD™ FungaLight™ Yeast Viability Kit, for flow cytometry

Invitrogen™ LIVE/DEAD™ FungaLight™ Yeast Viability Kit, for flow cytometry

The LIVE/DEAD® FungaLight™ Yeast Viability Kit enables researchers to easily, reliably and quantitatively distinguish live and dead yeast in minutes by flow cytometry. The kit contains solutions of the SYTO® 9 green-fluorescent nucleic acid stain and the red-fluorescent nucleic acid stain, propidium iodide. These stains differ both in their spectral characteristics and in their ability to penetrate healthy yeast cells. When used alone, the SYTO® 9 stain generally labels all yeast in a population - those with intact membranes and those with damaged membranes. In contrast, propidium iodide penetrates only yeast with damaged membranes, causing a reduction in the SYTO® 9 stain fluorescence by fluorescence resonance energy transfer (FRET) when both dyes are present. As a result, yeast with intact membranes stain fluorescent green, whereas yeast with damaged membranes stain fluorescent red.

Application: Viability Assay.

Content

• 1 vial SYTOfi 9 dye, 3.34 mM (Component A), 300 μL solution in DMSO.
• 1 vial Propidium iodide, 20 mM (Component B), 300 μL solution in DMSO.