Invitrogen™ APO-BrdU™ TUNEL Assay Kit, with Alexa Fluor™ 488 Anti-BrdU

Invitrogen™ APO-BrdU™ TUNEL Assay Kit, with Alexa Fluor™ 488 Anti-BrdU

In collaboration with Phoenix Flow Systems, we offer the APO™-BrdU TUNEL Assay Kit which provides all the materials necessary to label and detect the DNA strand breaks of apoptotic cells. When DNA strands are cleaved or nicked by nucleases, a large number of 3´hydroxyl ends are exposed. In the APO-BrdU assay, these ends are labeled with BrdUTP and terminal deoxynucleotidyl transferase (TdT) using the TUNEL technique. Once incorporated ino the DNA, BrdU is detected using a bright and photostable green-fluorescent Alexa Fluor™ 488 dye-labeled anti-BrdU antibody.

For Use With (Equipment): Fluorescence Microscope, Flow Cytometer

Features

• Excitation/Emission: 495⁄519
• Flow Cytometer Laser Lines: 488
• Host Species: Mouse
• Label Type: Alexa Fluor Dyes
• Label or Dye: Alexa Fluor™ 488
• Target Gene: BrdU
• Detection Method: Fluorescent

Contents

• Positive control cells (Component A, brown cap), fixed human lymphoma cell line: 5 mL
• Negative control cells (Component B, white cap), fixed human lymphoma cell line: 5 mL 
• Terminal deoxynucleotidyl transferase (Component C, yellow cap): 45 μL
• 5-Bromo-2’-deoxyuridine 5’-triphosphate (BrdUTP) (Component D, violet cap): 480 μL
• anti-BrdU Ms mAb Alexa Fluor™ 488 conjugate: 350 µL
• PI/Rnase A staining buffer: 30 mL
• Reaction buffer: 0.6 mL
• Wash buffer: 120 mL 
• Rinse buffer: 120 mL