Invitrogen™ Luc-Screen™ Extended-Glow Luciferase Reporter Gene Assay System, 200 Assays

Invitrogen™ Luc-Screen™ Extended-Glow Luciferase Reporter Gene Assay System, 200 Assays

The Luc-Screen® assay system with extended-glow light emission is designed for sensitive detection of firefly luciferase reporter enzyme in microplate cell cultures. A linear signal is obtained with the Luc-Screen® assay from 50 fg to 100 ng of pure enzyme in culture medium.

For use with eauipment: Luminometer (Microplate)

Features

• Homogeneous assay format allows detection of firefly luciferase in the presence of normal culture media without removal of media and without an additional cell lysis step, providing the easiest and most streamlined assay procedure possible.
• The glow assay kinetics provides a "window" during which measurements may be performed, facilitating HTS applications where assay automation is used.
• Wide dynamic range of firefly luciferase assay lets the user measure enzyme level accurately from femtogram to nanogram range.
• Assay sensitivity is 100- to 1,000-fold better than with either the isotopic⁄non-isotopic assays for chloramphenicol acetyl transferase (CAT) or the colorimetric⁄fluorescent assays for b-galactosidase, providing greater sensitivity than competing assay technologies.
• Highly sensitive assay with a wide dynamic range permits detection of high and low levels of reporter without performing numerous sample dilutions.
• Non-radioactive reporter gene assay kit eliminates radioisotopes.
• Assay can be completed in about one hour, providing fast assay turnaround. 

Contents

• This kit contains two reaction buffers.