Thermo Scientific™ Pierce™ HA-Tag IP/Co-IP Kit

Thermo Scientific™ Pierce™ HA-Tag IP/Co-IP Kit

The Thermo Scientific Pierce HA Tag IP/Co-IP Kit provides the affinity resin, positive control and other reagents necessary to perform immunoprecipitation (IP) or co-immunoprecipitation (Co-IP) reactions with an HA-tagged bait protein.

Features

• Improved—updated kit includes more immobilized antibody resin and improved protocol along with GST-PI3K-SH2-HA fusion protein as a positive control
• Specific—highly specific anti-HA monoclonal antibody provides high-yield immunoprecipitation products and clean Western blot detection
• Compatible—includes protocols and reagents for multiple elution conditions to accommodate different protein sensitivities and downstream applications
• Robust—the kit and the anti-HA agarose are compatible with various cell lysates and physiologic (non-denaturing) buffer systems
• Convenient and easy—complete kit includes all necessary reagents, convenient spin columns and easy-to-follow instructions

Contents

• Anti-HA Agarose: 0.25 mL
• HA-tagged Positive Control: 0.5 mL
• BupH Tris Buffered Saline Pack (500 mL): 1 pack
• Lane Marker Non-Reducing Sample Buffer (5X): 5 mL
• Pierce Spin Columns Accessory Pack: 27 columns
• Collection Tubes and Caps Accessory Pack: 100 tubes