Invitrogen Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Super Bright 645, 25 µg, eBioscience™

Invitrogen Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Super Bright 645, 25 µg, eBioscience™

The monoclonal mouse IgG1 K immunoglobulin is useful as an isotype control.

Applications: Control (Ctrl).

Features

• Super Bright 645 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 645 nm. We recommend using a 660/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.
• When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.
• Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Protect this vial and stained samples from light.
• Fixation: Samples can be stored in IC Fixation Buffer (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
• Excitation: 405 nm; Emission: 645 nm; Laser: Violet Laser.