Invitrogen Mouse IgG2a kappa Isotype Control (eBM2a), Super Bright™ 780, 100 µg, eBioscience™

Invitrogen Mouse IgG2a kappa Isotype Control (eBM2a), Super Bright™ 780, 100 µg, eBioscience™

This is a monoclonal mouse IgG2a, kappa antibody. It is used as an isotype control for mouse IgG2a antibodies.

Super Bright 780 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 780 nm. We recommend using a 780/60 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.
When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution for up to 3 days in the dark at 2-8°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorochrome performance after fixation can be made, but clone specific performance should be determined empirically.