Invitrogen Mouse IgG2b kappa Isotype Control (eBMG2b), Super Bright 780, 100 µg, eBioscience™

Invitrogen Mouse IgG2b kappa Isotype Control (eBMG2b), Super Bright 780, 100 µg, eBioscience™

The monoclonal mouse IgG2b, kappa is useful as an isotype control immunoglobulin.

Applications: Control (Ctrl).

Features  

• Super Bright 780 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 780 nm. We recommend using a 780/60 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.
• When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.
• Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
• Fixation: Samples can be stored in IC Fixation Buffer (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
• Excitation: 405 nm; Emission: 780 nm; Laser: Violet Laser.