Invitrogen™ MultiShot™ FlexPlate TOP10 Competent Cells

Invitrogen™ MultiShot™ FlexPlate TOP10 Competent Cells

MultiShot FlexPlate TOP10 chemically competent E. coli cells are cloning competent cells that are pre-aliquoted in a 96-well PCR plate to increase transformation productivity. The FlexPlate format can be divided down to any combination of 8-well segments to allow for flexibility in transformation throughput. MultiShot FlexPlate TOP10 competent E. coli cells are ideal for high-efficiency cloning and plasmid propagation. They allow stable replication of high-copy number plasmids and are the same competent cells that come with many of our cloning kits.

Features

• hsdR for efficient transformation of unmethylated DNA from PCR amplifications
• mcrA for efficient transformation of methylated DNA from genomic preparations
• lacZΔM15 for blue/white color screening of recombinant clones
• endA1 for cleaner preparations of DNA and better results in downstream applications due to the elimination of non-specific digestion by endonuclease I
• recA1 for reduced occurrence of non-specific recombination in cloned DNA

Contents

• MultiShot FlexPlate TOP10 Competent Cells: One 96-well plate, 20 µL per well
• pUC19 DNA (10 pg/µL): 1 vial, 50 µL
• S.O.C. medium: 2 bottles, 10 mL each