Invitrogen™ MultiShot™ FlexPlate Stbl3™ Competent Cells

Invitrogen™ MultiShot™ FlexPlate Stbl3™ Competent Cells

MultiShot FlexPlate Stbl3 chemically competent E. coli cells are cloning competent cells that are packaged in a rack containing 12 strips of 8 wells to increase productivity for medium-throughput bacterial transformations. MultiShot FlexPlate Stbl3 competent E. coli cells are designed especially for cloning direct repeats found in lentiviral expression vectors. Unlike TOP10 E. coli, these cells reduce the frequency of homologous recombination of long terminal repeats (LTRs) found in ViraPower Lentiviral Expression vectors and other retroviral vectors. The DNA yield from Stbl3 cells is often >10-fold higher than alternative strains such as TOP10

Features

• Optimal performance for the propagation of lentiviral vectors
• mcrB, mrr for enhanced genomic cloning capacity
• recA13 for reduced occurrence of non-specific recombination in cloned DNA
• High DNA yields

Contents

• MultiShot FlexPlate Stbl3 Competent Cells: One 96-well plate, 20 µL per well
• pUC19 DNA (10 pg/µL): 1 vial, 50 µL
• S.O.C. medium: 2 bottles, 10 mL each