Invitrogen™ Newport Green™ DCF Diacetate, cell permeant

The Newport Green™ DCF indicator has moderate zinc-binding affinity (K_d for Zn²⁺ ∼1 µM) but is essentially insensitive to Ca²⁺ (Kd for Ca²⁺ >100 µM), making this a valuable probe for detecting Zn²⁺ influx into neurons through voltage- or glutamate-gated channels. When used alongside dyes with dual Ca²⁺ / Zn²⁺ sensitivity such as fura-2 and mag-fura-2, Newport Green™ DCF provides confirmation that changes in Zn²⁺ levels, and not Ca²⁺ or Mg²⁺, are being detected. The response of Newport Green™ DCF to transition metals, including Zn, Mn, Fe, Co, Cu(I), Cu(II), Ni and Cd, has been characterized