Invitrogen™ Intracellular pH Calibration Buffer Kit
On demand
Order number :
P35379
Invitrogen™ Intracellular pH Calibration Buffer Kit
The Intracellular pH Calibration Buffer Kit enables the quantitation of
intracellular pH when used in conjunction with pHrodo™ Red AM, pHrodo™
Green AM, or BCECF intracellular pH indicators. pHrodo™ Red AM and
pHrodo™ Green AM are weakly fluorescent at neutral pH but increasingly
fluorescent as the pH drops. Modification of pHrodo™ red and green dyes
with AM ester groups results in an uncharged molecule that can permeate
cell membranes. Once inside the cell, the lipophilic blocking groups are
cleaved by nonspecific esterases, resulting in a compound that is
retained within the intracellular space. Fluorescence intensity of the
probes is then an indicator of intracellular pH. Subsequent use of the
Intracellular pH Calibration Buffer Kit allows this intracellular pH to
be quantified
Features
- For Use With (Equipment): Fluorescence Microscope, Flow Cytometer, Fluorometer, High Content Instrument
- Sub Cellular Localization: Cytosol
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