Invitrogen™ pHrodo™ Deep Red Mammalian and Bacterial Cell Labeling Kit

Invitrogen™ pHrodo™ Deep Red Mammalian and Bacterial Cell Labeling Kit

The pHrodo Deep Red Mammalian and Bacterial Cell Labeling Kit provides the reagents you need to easily label live cells (mammalian or bacterial) for the study of phagocytosis and endocytosis. It is optimized for the discrimination of phagocytosed and non-phagocytosed cells since pHrodo Deep Red dye only fluoresces upon exposures to acidic environments. The low-background pHrodo Deep Red labeling kit makes it easier than ever to study endocytosis and phagocytosis with higher certainty of results and less optimization since pHrodo Deep Red dye only fluoresces in the late endosome and lysosome

Features

• Multiplex—Cy5 channel allows for multicolor experiments
• Simple—easy-to-follow step-by-step protocol
• Superior signal window—minimal background at neutral pH enables maximal signal-to-noise ratio for certainty of results
• Low pKa—eliminates non-specific signal from non-internalized cargo and only fluoresces in late endosomes and lysosomes

Contents

• pHrodo™Deep Red Cell Labeling Dye (Component A), MW ~1,300 g/mol
• Cell Labeling and WashBuffer(Component B)