Invitrogen™ pHrodo™ Green Maleimide

Invitrogen™ pHrodo™ Green Maleimide

The thiol-reactive pH-sensitive pHrodo® Green Maleimide dye is suitable for the creation of bioconjugates to study endocytosis and phagocytosis. pHrodo® Green dramatically increases fluorescence as the pH of its surroundings become more acidic.

• Use pH-sensitive pHrodo® Green Maleimide to make pH-sensitive bioconjugates of your choice

• Get faster, more accurate results than with any other endocytosis or phagocytosis assay—no need for wash steps or quenchers

• Multiplex with red fluorescent markers such as RFP, pHrodo® Red, and many others

The increase in fluorescence of pHrodo® Green as pH changes from basic to acidic correlates with the acidification of intracellular vesicles, making it an ideal tool to study endocytosis or phagocytosis and their regulation by environmental factors, drugs, or pathogens. The spectral properties of pHrodo® Green makes it useful for multi-color experiments. pHrodo® Green can be detected using most standard green fluorescent filter sets and has been validated for use on a variety of platforms including flow cytometry, fluorescent microscopy, and high content screening (HCS). The lack of fluorescence of pHrodo® Green in a typical extracellular environment eliminates the need for wash steps or quencher dyes in the experimental workflow.