Thermo Scientific™ Pierce™ SATA (N-succinimidyl S-acetylthioacetate)
On demandThermo Scientific™ Pierce™ SATA (N-succinimidyl S-acetylthioacetate)
Thermo Scientific Pierce SATA is a short-chain (2.8 angstrom spacer arm) reagent for covalent modification of primary amines and addition of a protected yet exposable sulfhydryl group, enabling heterobifunctional crosslinking strategies.
SATA (N-succinimidyl S-acetylthioacetate) adds sulfhydryl groups to proteins and other amine-containing molecules in a protected form. The modified molecule can be stored indefinitely and treated with hydroxylamine to expose the labile sulfhydryl group when needed for conjugation reactions. SATA contains an N-hydroxysuccinimide (NHS) ester, which forms a stable, covalent amide bond with primary amines (i.e., lysine residues and the amino termini of proteins) and releases NHS as a by-product. De-protection (deacylation) to generate a free sulfhydryl is accomplished using hydroxylamine-HCl.
Features
- Adds a protected sulfhydryl that can be deprotected by hydroxylamine
- Allows long-term storage of the sulfhydryl-modified molecule
- Forms cleavable disulfide bonds with other sulfhydryl-containing molecules
- Reacts with primary amines (e.g., lysine residues proteins) to form stable amide bonds
- Preserves protein activity with its mild, non-denaturing reaction conditions
There are no specifications
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