Invitrogen™ PrestoBlue™ HS Cell Viability Reagent, 25 mL
On demand
Order number :
P50200
Invitrogen™ PrestoBlue™ HS Cell Viability Reagent, 25 mL
PrestoBlue HS Cell Viability Reagent is a ready-to-use resazurin-based
solution that functions as a cell health indicator by using the reducing
power of living cells to quantitatively measure viability. Upon
entering living cells, resazurin is reduced to resorufin, a compound
that is red in color and highly fluorescent. High background
fluorescence caused by various contaminants reduces the performance of
resazurin-based reagents. To improve the performance an innovative
purification process was developed that removes these contaminants. This
highly purified, non-toxic resazurin was used in the standard
PrestoBlue formulation, creating the PrestoBlue HS Cell Viability
Reagent. Changes in viability can be easily detected using either an
absorbance- or fluorescence-based plate reader. The PrestoBlue HS Cell
Viability Reagent has been optimized for the fast and reliable detection
of mammalian cell viability
Features
- Removal of contaminants from resazurin—displays a >50% reduction in background fluorescence
- Signal-to-background ratio increased by >100%—results in large assay signal window
- Highly sensitive reagent with a linear response—detects as few as 10 cells per well
- Convenient add-and-read format—no mixing, no washing, no cell lysis, and compatible with either fluorescence- or absorbance-based instrumentation
- Optimized for reliable detection of mammalian cell viability in only 10 minutes
- For Use With (Equipment): Microplate Reader, Spectrophotometer, HTS Reader
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