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Invitrogen™ PrestoBlue™ HS Cell Viability Reagent, 25 mL

On demand
Order number : P50200
(Ex-work price)
$ On demand

Invitrogen™ PrestoBlue™ HS Cell Viability Reagent, 25 mL


PrestoBlue HS Cell Viability Reagent is a ready-to-use resazurin-based solution that functions as a cell health indicator by using the reducing power of living cells to quantitatively measure viability. Upon entering living cells, resazurin is reduced to resorufin, a compound that is red in color and highly fluorescent. High background fluorescence caused by various contaminants reduces the performance of resazurin-based reagents. To improve the performance an innovative purification process was developed that removes these contaminants. This highly purified, non-toxic resazurin was used in the standard PrestoBlue formulation, creating the PrestoBlue HS Cell Viability Reagent. Changes in viability can be easily detected using either an absorbance- or fluorescence-based plate reader. The PrestoBlue HS Cell Viability Reagent has been optimized for the fast and reliable detection of mammalian cell viability


Features

  • Removal of contaminants from resazurin—displays a >50% reduction in background fluorescence
  • Signal-to-background ratio increased by >100%—results in large assay signal window
  • Highly sensitive reagent with a linear response—detects as few as 10 cells per well
  • Convenient add-and-read format—no mixing, no washing, no cell lysis, and compatible with either fluorescence- or absorbance-based instrumentation
  • Optimized for reliable detection of mammalian cell viability in only 10 minutes
  • For Use With (Equipment): Microplate Reader, Spectrophotometer, HTS Reader
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