Invitrogen™ SYTOX™ Deep Red Nucleic Acid Stain, for fixed/dead cells, 50 µL

Invitrogen™ SYTOX™ Deep Red Nucleic Acid Stain, for fixed/dead cells, 50 µL

SYTOX Deep Red Nucleic Acid Stain is a high-affinity nucleic acid stain that easily penetrates cells with compromised plasma membranes and yet does not cross the membranes of live cells. With minimal cytoplasmic staining, SYTOX Deep Red stain is particularly useful in immunocytochemistry (ICC), immunohistochemistry (IHC), and immunofluorescence (IF) experiments for staining of nuclei of fixed cells. After a brief incubation with SYTOX Deep Red stain, the nucleic acids of dead or fixed cells fluoresce a bright, deep red/far red and can be detected with a CY5/Deep Red standard filter set or laser configuration

Features

• Impermeant to live cells; permeant to dead or fixed cells
• Excitation/emission peak: 660/682 nm
• Use with Cy5 traditional filter set or 647 laser line
• Works with mammalian cells in mono layer or 3D cell culture, animal tissue, and bacteria
• Detectable with fluorescence micro¬scopes, fluorimeters, fluorescence microplate readers, and flow cytometers
• For Use With (Equipment): Fluorescence Microscope

Contents

• 1 vial with 50 µL at 1 mM (2000 X) concentration in DMSO.Each vial contains enough reagent to stain ~1,000 samples when 100 µL/sample is used on the slide or in microplate at 1 x (0.5 µM) concentration