Invitrogen Prolactin Human ELISA Kit, 5 x 96 Tests

Invitrogen Prolactin Human ELISA Kit, 5 x 96 Tests

The Human Prolactin ELISA quantitates human prolactin in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Prolactin.
Principle of the method
The Human Prolactin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Features  

• Homogenous (no wash): No    
• Interassay CV: 10.8%
• Intraassay CV: 7.6%
• Shipping condition: Wet or Dry Ice
• Storage: -20°C

Contents    

• Prolactin Standard; 500 pg recombinant human prolactin, store at –20°C, 2 vials
• Assay Buffer Concentrate (5X), 28 mL
• Prolactin Antibody Coated Wells, 96-well strip-well plate,1 plate
• Prolactin Conjugate, store at –20°C, 5 mL
• Wash Buffer Concentrate (20X), 30 mL
• Tetramethylbenzidine (TMB) Substrate,11 mL
• Stop Solution; c ontains 1 M HCl, CAUSITC, 5 mL
• Plate Sealer, 1