Invitrogen™ PureLink™ RNA Mini Kit, 50 Preps

Invitrogen™ PureLink™ RNA Mini Kit 50 preps

The PureLink RNA Mini Kit is a column-based kit used to isolate high-quality total RNA from a wide variety of sample types in 20 minutes using standard laboratory equipment. The kit includes RNase-free lysis and wash solutions that protect RNA from RNases while liberating the RNA from DNA, proteins, and other cellular debris. The fast spin-column workflow is ideal for processing low to mid-throughput batch sizes. The advanced PureLink RNA Mini spin column design allows for maximum sample input (200 mg of tissue) and RNA recovery (up to 1000 µg). This means that users can process both small and large sample sizes with the same RNA isolation kit.

Features

• Process sample inputs from 5–200 mg of animal/plant tissue or from 0.5 x 106 to 108 tissue culture cells with one spin column in 20 minutes
• Isolate more RNA than competitor mini spin columns: up to 1000 µg of total RNA from one column
• Use isolated RNA in most applications without the need for further DNase treatment (very low minimal residual DNA carry over)
• Flexible–optional on-column or post-isolation DNase treatment is available with PureLink DNase Set for easy removal of residual DNA
  
  The PureLink RNA Mini Kit is recommended for use with the Homogenizer designed to homogenize cell or tissue lysates via centrifugation, prior to nucleic acid purification. The Homogenizer is especially effective for clarifying particulates from plant tissues.

Contents

• Lysis Buffer: 125 mL
• Wash Buffer I: 50 mL
• Wash Buffer II: 15 mL
• RNase-Free Water: 15.5 mL
• Spin Cartridges with collection tubes: 50 each
• Collection Tubes: 50 each
• Recovery Tubes: 50 each