Invitrogen™ Quant-iT™ RNA Quantitation, Extended Range, 1000 Assays

Invitrogen™ Quant-iT™ RNA Quantitation, Extended Range, 1000 Assays

The Quant-iT RNA XR (Extended Range) Assay Kit provides an accurate and selective method for the quantitation of high-abundance RNA samples. The assay is highly selective for RNA and does not quantitate DNA, protein, or free nucleotides. Common contaminants, such as salts, free nucleotides, solvents, detergents, and protein, are well-tolerated in the assay. The assay kit is designed to be accurate for initial RNA sample concentrations of 10 ng/µL to 10,000 ng/µL depending on sample volume, providing a linear detection range of 200–10,000 ng. The kit provides concentrated assay reagent, dilution buffer, and pre-diluted RNA standards. Simply dilute the reagent using the buffer provided, add the sample (any volume between 1 µL and 20 µL is acceptable), and read the concentration using a fluorescent-based microplate reader.

The Quant-iT RNA XR (Extended Range) Assay Kit provides an accurate and selective method for the quantitation of high-abundance RNA samples. The assay is highly selective for RNA and does not quantitate DNA, protein, or free nucleotides. The assay kit is designed to be accurate for initial RNA sample concentrations of 10 ng/µL to 10,000 ng/µL depending on sample volume, providing a linear detection range of 200–10,000 ng. The kit provides concentrated assay reagent, dilution buffer, and pre-diluted RNA standards. Simply dilute the reagent using the buffer provided, add the sample (any volume between 1 µL and 20 µL is acceptable), and read the concentration using a fluorescent-based microplate reader.

Features

• Accurately detects as little as 2 ng RNA in a microplate well
• Core dynamic range of 5–100 ng
• Approximate fluorescence excitation/emission maxima of 644/673 nm

Contents

• Quant-iT™ RNA XR Reagent (Component A): 1 mL
• Quant-iT™ RNA XR Buffer (Component B): 225 mL
• Yeast tRNA Standards (Component C): Set of 8 (500 μL each)