Invitrogen™ Ulysis™ Alexa Fluor™ 647 Nucleic Acid Labeling Kit

Invitrogen™ Ulysis™ Alexa Fluor™ 647 Nucleic Acid Labeling Kit

ULYSIS® Nucleic Acid Labeling Kits provide a unique method to attach a fluorescent dye to nucleic acids. The labeling reagent in the kit reacts with the N7 of guanine to form a stable coordination complex, and the reaction is simple and fast—just heat denature DNA (5 minutes), add the label (react for 15 minutes), then purify.
We developed this series of ULYSIS® kits to enable rapid and simple coupling of our Alexa Fluor® dyes to purine bases in nucleic acid polymers. The method, the Universal Linkage System (ULS™), is based on the use of a platinum dye complex (owned by KREATECH Diagnostics) that forms a stable adduct with the N7 position of guanine and, to a lesser extent, adenine bases in DNA, RNA, PNA, and oligonucleotides. The result is a reliable nonenzymatic method for nucleic acid labeling.
The Resulting Labeled Probe is useful for:

• Dot, northern, and Southern blots
• RNA and DNA in situ hybridization
• Multicolor fluorescence in situ hybridization (mFISH)
• Comparative genome hybridization (CGH)
• Microarray analysis

Features

• Dye (Ex/Em): Alexa Fluor® 647 (650/670 nm)
• Labeling reaction is complete in as little as 15 minutes
• Available in several Alexa Fluor® dye colors

Contents

• ULS® labeling reagent (Component A): 1 vial for 20 labelings or 5 vials for 4 labelings each (U21650)
• Dimethylformamide DMSO) (Component B): 200 μL
• Labeling buffer (Component C): 600 μL
• Deoxyribonuclease I (DNase I) (Component D): 100 μg
• DNase I storage buffer (Component E): 200 μL
• 10X DNase I reaction buffer (Component F): 500 μL
• DNA from calf thymus (Component G): 100 μL
• Nuclease-free H2O (Component H): 5 mL