Invitrogen™ QuantiGene™ Plex Assay Kit, 3 Plates

Invitrogen™ QuantiGene™ Plex Assay Kit, 3 Plates

The QuantiGene Plex Assay Kit contains the generic reagents, buffers, and plates necessary to perform the assay in 96-well plates, and must be used with a target-specific QuantiGene Plex panel (sold separately).
The QuantiGene Plex Assay is a gene expression assay that enables multiplexed measurement of up to 80 RNA transcript targets. The assay uses target-specific probes to capture the RNA of interest, and branched DNA technology then amplifies fluorescent signals which are read with a Luminex xMAP instrument.

Features

• Difficult sample types—works with degraded and cross-linked RNA in FFPE tissues and blood
• True multiplexing—measure up to 80 genes of interest and housekeeping genes in the same well with no cross-reactivity
• Standardized platform—uses 96 or 384-well plate formats compatible with Luminex 200, MagPix, and FlexMap3D systems
• Simple workflow—employs an ELISA-like workflow for direct hybridization of transcripts to beads and transcript labeling
• Large inventory of validated genes—select from over 15,000 genes to create pathway and disease-themed panels
• Rapid turnaround for custom panels—if we don’t have your gene(s), we can create a custom panel within two weeks

Applications

QuantiGene Plex assay applications include compound screening of drug candidates, biomarker verification, siRNA knockdown efficiency, prospective and/or retrospective analysis of clinical samples, miRNA profiling, microarray validation, predictive toxicology, and detection of translocations and fusion genes.

Contents

• Proteinase K: 105 µL
• Blocking Reagent: 810 µL
• Label Probe Solution: 36 mL
• Pre-Amplifier Solution: 36 mL
• Amplifier Solution: 36 mL
• SAPE: 108 µL
• SAPE Diluent: 36 mL
• Lysis Mixture: 36 mL
• Wash Buffer Component 1: 2.4 mL
• Wash Buffer Component 2: 39 mL
• SAPE Wash Buffer: 420 mL
• Magnetic Separation Plates: 3
• Plate Seals: 30
• Hybridization Plates: 3
• Pressure Seals: 6