Invitrogen™ QuantiGene™ Plex Assay Kit, 1 Plate
On demandInvitrogen™ QuantiGene™ Plex Assay Kit, 1 Plate
The QuantiGene Plex Assay Kit contains the generic reagents, buffers, and plates necessary to perform the assay in 96-well plates, and must be used with a target-specific QuantiGene Plex panel (sold separately).
The QuantiGene Plex Assay is a gene expression assay that enables multiplexed measurement of up to 80 RNA transcript targets. The assay uses target-specific probes to capture the RNA of interest, and branched DNA technology then amplifies fluorescent signals which are read with a Luminex xMAP instrument.
Features
- Difficult sample types—works with degraded and cross-linked RNA in FFPE tissues and blood
- True multiplexing—measure up to 80 genes of interest and housekeeping genes in the same well with no cross-reactivity
- Standardized platform—uses 96 or 384-well plate formats compatible with Luminex 200, MagPix, and FlexMap3D systems
- Simple workflow—employs an ELISA-like workflow for direct hybridization of transcripts to beads and transcript labeling
- Large inventory of validated genes—select from over 15,000 genes to create pathway and disease-themed panels
- Rapid turnaround for custom panels—if we don’t have your gene(s), we can create a custom panel within two weeks
Applications
QuantiGene Plex assay applications include compound screening of drug candidates, biomarker verification, siRNA knockdown efficiency, prospective and/or retrospective analysis of clinical samples, miRNA profiling, microarray validation, predictive toxicology, and detection of translocations and fusion genes.
Contents
- Proteinase K: 37 µL
- Blocking Reagent: 270 µL
- Label Probe Solution: 12 mL
- Pre-Amplifier Solution: 12 mL
- Amplifier Solution: 12 mL
- SAPE: 36 µL
- SAPE Diluent: 12 mL
- Lysis Mixture: 12 mL
- Wash Buffer Component 1: 0.8 mL
- Wash Buffer Component 2: 13 mL
- SAPE Wash Buffer: 140 mL
- Magnetic Separation Plates: 1
- Plate Seals: 10
- Hybridization Plates: 1
- Pressure Seals: 2
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