Thermo Scientific™ DRAQ5™ Fluorescent Probe Solution (5 mM), 1 mL

Thermo Scientific™ DRAQ5™ Fluorescent Probe Solution (5 mM), 1 mL

The Thermo Scientific DRAQ5™ Fluorescent Probe is a far-red DNA stain for live or fixed cells and easy multiplexing with other fluorophores, such as cells expressing green fluorescent protein (GFP) fusion proteins.
DRAQ5™ Fluorescent Probe is a far-red DNA stain for fluorescent cellular imaging applications with live or fixed cells. Because of its far-red excitation and emission, the DRAQ5 Stain can be multiplexed with many other fluorophores. It is commonly chosen to counterstain nuclei and measure DNA content in cell proliferation studies involving cells expressing green fluorescent protein (GFP) fusion proteins. DRAQ5 Fluorescent Probe is compatible with many existing protocols across a wide range of instrumentation platforms. Fluorescence cell-based assays, such as flow cytometry, in-cell ELISA, fluorescence microscopy and high-content imaging require a fluorescent label to identify individual cells. When using multiple fluorescent probes to detect different cellular targets or activities, each probe must have a fluorescent spectrum different than the other probes. The blue-fluorescent DNA-binding probes, Hoechst and DAPI are frequently used; however, these probes cannot be used when UV illumination is unavailable or other blue-emitting fluorescent probes are used. Therefore, nuclear probes that emit in a color other than blue are useful for cell identification and counting, and for determining nuclear morphology and DNA content

Features

• DRAQ5 dye—a red-to-infrared fluorescent stain specific for DNA (nuclei and DNA quantitation)
• Convenient—provided as easy-to-use DRAQ5 dye solutions (5 mM)
• Cellular imaging—lipophilic and membrane permeable dye is effective for live-cell and fixed-cell staining and quantitation of DNA content
• Counterstain—ideal for use alongside other fluorophore reporters for fluorescence microscopy or high-content screening (HCS)
• Sub Cellular Localization: Nucleus, Cytosol, Cytoplasm
• For Use With (Equipment): Fluorescence Microscope, High Content Instrument