Thermo Scientific™ DRAQ5™ Fluorescent Probe Solution (5 mM), 1 mL
On demandThermo Scientific™ DRAQ5™ Fluorescent Probe Solution (5 mM), 1 mL
The
Thermo Scientific DRAQ5™ Fluorescent Probe is a far-red DNA stain for
live or fixed cells and easy multiplexing with other fluorophores, such
as cells expressing green fluorescent protein (GFP) fusion proteins.
DRAQ5™
Fluorescent Probe is a far-red DNA stain for fluorescent cellular
imaging applications with live or fixed cells. Because of its far-red
excitation and emission, the DRAQ5 Stain can be multiplexed with many
other fluorophores. It is commonly chosen to counterstain nuclei and
measure DNA content in cell proliferation studies involving cells
expressing green fluorescent protein (GFP) fusion proteins. DRAQ5
Fluorescent Probe is compatible with many existing protocols across a
wide range of instrumentation platforms. Fluorescence cell-based assays, such as flow cytometry, in-cell ELISA,
fluorescence microscopy and high-content imaging require a fluorescent
label to identify individual cells. When using multiple fluorescent
probes to detect different cellular targets or activities, each probe
must have a fluorescent spectrum different than the other probes. The
blue-fluorescent DNA-binding probes, Hoechst and DAPI
are frequently used; however, these probes cannot be used when UV
illumination is unavailable or other blue-emitting fluorescent probes
are used. Therefore, nuclear probes that emit in a color other than blue
are useful for cell identification and counting, and for determining
nuclear morphology and DNA content
Features
- DRAQ5 dye—a red-to-infrared fluorescent stain specific for DNA (nuclei and DNA quantitation)
- Convenient—provided as easy-to-use DRAQ5 dye solutions (5 mM)
- Cellular imaging—lipophilic and membrane permeable dye is effective for live-cell and fixed-cell staining and quantitation of DNA content
- Counterstain—ideal for use alongside other fluorophore reporters for fluorescence microscopy or high-content screening (HCS)
- Sub Cellular Localization: Nucleus, Cytosol, Cytoplasm
- For Use With (Equipment): Fluorescence Microscope, High Content Instrument
There are no specifications
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