Invitrogen™ S1 Nuclease

Invitrogen™ S1 Nuclease

S1 Nuclease is a single-strand-specific endonuclease that hydrolyzes single-stranded RNA or DNA into 5´ mononucleotides. The enzyme will hydrolyze single-stranded regions in duplex DNA such as loops and gaps. S1 Nuclease is stable at 65°C.

Applications: Nuclease mapping techniques. Removal of single-stranded regions from double-stranded DNA. Exo III-ordered sequencing.

Source: Isolated from Aspergillus oryzae.

Contents

• S1 Nuclease is supplied with a vial of 10X S1 Nuclease buffer [300 mM sodium acetate (pH 4.6), 10 mM zinc acetate, 50% (v/v) glycerol], vial of dilution buffer, vial of 3 M NaCl. Store at -20°C.