Invitrogen™ SAIVI™ Alexa Fluor™ 647 Antibody/Protein 1 mg-Labeling Kit

Invitrogen™ SAIVI™ Alexa Fluor™ 647 Antibody/Protein 1 mg-Labeling Kit

The SAIVI Alexa Fluor 647 Antibody/Protein Labeling Kit provides the materials needed conveniently label an antibody or protein with amine-reactive far-red fluorescent Alexa Fluor 647 dye (ex/em 650/665 nm) for in vivo animal imaging applications. This kit is optimized for labeling 1 mg of IgG antibody per reaction with Alexa Fluor 647 and comparably small amounts of other proteins (>20 kDa) can also be labeled. Each kit contains everything needed for three labeling reactions, degree of labeling (DOL) modification, and subsequent purification for free dye removal. Our protein labeling kits include an easy-to-use pre-packed spin column for rapid dye removal and high antibody recovery typically greater than 85%. Stabilizing proteins must be removed from the sample before labeling.

Features

• Convenient—labeled proteins typically ready to use in 90 min (∼15 min hands-on time)
• High conjugate recovery—pre-packed spin columns generally result in >85% recovery of antibody conjugate
• Simple protocol—react, separate, use; detailed instructions included for DOL determination and modification
• Rapid purification—quickly remove unbound dye with included pre-packed spin columns in under 15 minutes