Thermo Scientific™ Pierce™ Conjugate Purification Kit

Thermo Scientific™ Pierce™ Conjugate Purification Kit

The Thermo Scientific Pierce Conjugate Purification Kit is a gentle affinity chromatography system for purifying horseradish peroxidase (HRP) or alkaline phosphatase (AP) conjugated antibodies from unreacted enzyme after labeling.

This conjugate purification kit is designed to eliminate excess, unconjugated HRP or Alk-Phos enzyme from labeled antibodies (IgG) after performing antibody-enzyme conjugation reactions. The column of metal-chelate agarose resin binds to IgG-enzyme conjugates but allows non-conjugated enzyme (HRP or AP) to to pass through. A gentle, non-denaturing elution buffer recovers the fully active IgG-HRP or IgG-AP conjugate. The included desalting columns exchange the purified conjugate into any buffer of choice, ready for use in various immunodetection methods.

Features

• Specific for IgG—procedure is designed for removal of HRP or AP enzymes from IgG antibodies; not for use with IgM, IgY, Fab fragments or streptavidin conjugates
• Complete kit—includes affinity column, activator and elution buffers, desalting column and storage buffers for HRP and AP conjugates
• High capacity—the single-use column-kit is designed for purification of 0.5 to 50 mg of antibody conjugate dissolved in Tris-buffered saline
• Improves assays—removing unconjugated enzyme from antibody conjugates often reduces background and improves overal performance of immunoassays

Contents

Sufficient For: 10 purifications, each of 0.5 to 50 mg of enzyme-conjugated antibody

• IDA Agarose Column, 2 mL, 1 column
• Polyacrylamide Desalting Column, 10 mL, 1 column
• Column Activator Buffer, 25 mL
• Elution Buffer, 35 mL
• TBS Packs (each makes 500 mL), 2 packs
• PBS Pack (makes 500 mL), 1 pack
• Activity Preservation Solution, 1 mL