Invitrogen™ Single Cell-to-CT™ qRT-PCR Kit Green Features, 400 Reactions

Invitrogen™ Single Cell-to-CT™ qRT-PCR Kit, 400 Reactions

The Ambion® Single Cell-to-CT™ Kit contains a complete validated workflow for gene expression analysis for samples containing 1–10 cells. Each kit contains reagents for sample preparation, reverse transcription, pre-amplification, and qPCR that have been optimized together in a simple workflow that can be completed in only 5 steps .

Features

• Pre-optimized workflow for real-time RT-PCR from single cells
• Maximum sensitivity for single-cell analysis
• Superior performance and reproducibility compared to alternative methods
• Complete kit convenience and easy-to-follow protocol
• Green Features Fewer resources used and less waste, Less hazardous
• Detection Method Primer-Probe Detection
• PCR Method 2-Step qRT-PCR

Contents

• Single Cell DNase I:  400 µL
• Single Cell Lysis Solution: 4.0 mL
• Single Cell Stop Solution:  400 µL
• Single Cell SuperScript™ RT:  600 µL
• TaqMan™ Gene Expression MasterMix:   50 mL
• Single Cell Pre-Amp Mix:   2.1 mL
• Single Cell VILO™ RT Mix: 1.2 mL