Applied Biosystems™ PureQuant CD4+ T Cell Assay

Applied Biosystems™ PureQuant CD4+ T Cell Assay

The Applied Biosystems PureQuant assays are a family of assays that accurately identify and quantify specific immune cell types in a mixed population of cells. These assays are TaqMan qPCR-based and measure genomic DNA methylation at specific sites that serve as a unique identifier. The assays include standards and controls and each 96-well plate can accommodate up to seven test samples. Results are provided as a percentage of target cell type out of total input cells using a free analysis tool.

For use with equipments: QuantStudio™

Features

• Proven performance―core technology has been used for identification and quantification of immune cell types for over a decade
• Easily standardized―qPCR method enables consistent results across multiple users, instruments, and sites
• Facilitates regulatory filings―established performance specifications following ICHQ2(R1) guidelines
• Scheduling flexibility―assay can be performed using fresh or frozen cells/gDNA

Contents

• Dynabeads SILANE Genomic DNA Kit, store at 2–8°C
• 1 x 2 mL PureQuant qPCR Master Mix, store at 2–8°C
• 4 x ABS • THFA, store at room temperature
• PureQuant CD4 Module, store at -15 to -25°C (CD4 & GAPDH TaqMan primers and probes, Reference DNA, Lambda DNA, Standard DNA)